It is a 3-in-1 test, that allows doctors to tell if an individual is currently infected with chikungunya, dengue, or Zika viruses by using a single sample. The CDC Trioplex Real-time RT-PCR Assay is a diagnostic tool for Zika virus. The video shows scientists from the Centers for Disease Control and Prevention assembling CDC Trioplex Real-time RT-PCR Assay pdf icon external icon kits for shipment to qualified public health labs in the Laboratory Response Network and abroad.
BSL-3 and BSL-4 Laboratory Training: 1:32 Seconds. Rooms are under negative pressure relative to the outside areas so that any leak that develops will draw clean air into the laboratory without letting contaminated air escape. The air flow in a BSL-4 laboratory is unidirectional (outside to inside), ‘single pass’ (not recirculated), and filtered. Staff in BSL-4 suit laboratories must wear positive-pressure suits with an air supply and use chemical showers to disinfect the suit before leaving the laboratory. Scientists must use BSL-4 laboratories to work with pathogens that cause life-threatening diseases and require the highest level of safety and security, such as those which have no vaccines or treatments. In BSL-3 laboratories, scientists work with agents that may cause serious or potentially lethal diseases. The facility allows scientists to train at all four biosafety levels, and includes two mock high-containment laboratory settings, Biosafety Level 3 (BSL-3) and Level 4 (BSL-4). 
In this facility, scientists can train on required safety measures without the presence of infectious agents. This b-roll shows CDC’s newest laboratory training facility, where laboratory scientists prepare for future work with infectious agents.
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